{"id":16750,"date":"2025-01-31T08:30:00","date_gmt":"2025-01-31T07:30:00","guid":{"rendered":"https:\/\/adntro.com\/?p=16750"},"modified":"2025-11-19T17:02:06","modified_gmt":"2025-11-19T16:02:06","slug":"replicacion-adn","status":"publish","type":"post","link":"https:\/\/adntro.com\/en\/blog\/learn-genetics\/replication-adn\/","title":{"rendered":"DNA replication"},"content":{"rendered":"<p class=\" translation-block\">Our cells are constantly changing due to a series of events that take place during the <a href=\"https:\/\/adntro.com\/en\/blog\/aprende-genetica\/fases-ciclo-celular\/\" target=\"_self\">phases of the cell cycle<\/a>. Among these events, <a href=\"https:\/\/adntro.com\/en\/blog\/aprende-genetica\/replicacion_transcripcion_traduccion\/\" target=\"_self\">DNA replication<\/a> stands out, as it is <b>essential for cells to renew themselves<\/b>.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">What does cellular renewal consist of?<\/h2>\n\n\n\n<p class=\" translation-block\">Cellular renewal is the ability of cells to reproduce and <b>produce identical cells that replace the originals<\/b>. This process is responsible for maintaining our cells and is a continuous process.<\/p>\n\n\n\n<p class=\" translation-block\">One of the <b>key steps<\/b> in this process is <b>DNA replication<\/b>, during which the cell prepares its cellular machinery to make an exact copy of all its genetic material.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">How is DNA replication carried out in human cells?<\/h2>\n\n\n\n<p class=\" translation-block\">Human cells belong to the group of eukaryotic cells. These cells contain linear <a href=\"https:\/\/adntro.com\/en\/blog\/aprende-genetica\/que-es-adn\/\" target=\"_self\">DNA<\/a> in the form of a double helix. The double helix consists of two complementary DNA strands held together by hydrogen bonds. Both strands run in opposite directions, with one strand oriented 5\u2019 \u2192 3\u2019 (leading strand) and the other oriented 3\u2019 \u2192 5\u2019 (lagging strand).<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"104\" height=\"340\" src=\"https:\/\/adntro.com\/wp-content\/uploads\/2025\/01\/doble_helice_sentidos.png\" alt=\"DNA helix\" class=\"wp-image-16751\" srcset=\"https:\/\/adntro.com\/wp-content\/uploads\/2025\/01\/doble_helice_sentidos.png 104w, https:\/\/adntro.com\/wp-content\/uploads\/2025\/01\/doble_helice_sentidos-92x300.png 92w, https:\/\/adntro.com\/wp-content\/uploads\/2025\/01\/doble_helice_sentidos-4x12.png 4w\" sizes=\"(max-width: 104px) 100vw, 104px\" \/><\/figure>\n<\/div>\n\n\n<p>To create a copy of DNA, the following events must occur:<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li class=\" translation-block\">The cellular machinery must access the interior of the double helix to read the information we want to copy. To achieve this, the enzyme <b>helicase<\/b> breaks the hydrogen bonds that hold the two DNA strands together, creating a structure called the <b>replication fork<\/b>.<\/li>\n\n\n\n<li class=\" translation-block\">To relieve the tension in the DNA strands caused by the supercoiling of the double-helix structure, <b>topoisomerase<\/b> cuts and reattaches fragments of the DNA strand being replicated. We can imagine it as a structure responsible for unwinding the double helix.<\/li>\n\n\n\n<li class=\" translation-block\"><b>SSB proteins<\/b> bind to the DNA strand to stabilize it and prevent it from rejoining its complementary strand, as this would unable DNA replication.<\/li>\n\n\n\n<li class=\" translation-block\">The enzyme <b>primase<\/b> generates an RNA fragment (primer) complementary to the DNA segment that is to be replicated. This fragment is essential for DNA replication to begin. The primer provides a free 3\u2019 OH end, which serves as the binding site for the enzyme responsible for copying the information, DNA polymerase<\/li>\n\n\n\n<li class=\" translation-block\"><b>DNA polymerases<\/b> bind to the free 3\u2019 OH end of the primer and add complementary nucleotides to the DNA strand that is being copied.<\/li>\n<\/ol>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img fetchpriority=\"high\" decoding=\"async\" width=\"567\" height=\"395\" src=\"https:\/\/adntro.com\/wp-content\/uploads\/2025\/01\/dna_replication.png\" alt=\"DNA replication\" class=\"wp-image-16752\" srcset=\"https:\/\/adntro.com\/wp-content\/uploads\/2025\/01\/dna_replication.png 567w, https:\/\/adntro.com\/wp-content\/uploads\/2025\/01\/dna_replication-300x146.png 300w, https:\/\/adntro.com\/wp-content\/uploads\/2025\/01\/dna_replication-18x9.png 18w\" sizes=\"(max-width: 567px) 100vw, 567px\" \/><\/figure>\n<\/div>\n\n\n<p class=\" translation-block\">DNA polymerases only work in the <b>5\u2019 \u2192 3\u2019 direction<\/b>. In practical terms, this means that for the leading strand, a single primer is sufficient for the DNA polymerases to perform their function, while the lagging strand will require multiple primers to replicate the entire strand.<\/p>\n\n\n\n<p class=\" translation-block\">This creates replicated DNA fragments on the lagging strand known as <b>Okazaki fragments<\/b>. In the case of the lagging strand, the enzyme <b>ligase<\/b> is required to complete replication by \"joining\" these fragments together.<\/p>","protected":false},"excerpt":{"rendered":"<p>Our cells are constantly changing due to a series of events that occur during the phases of the cell cycle. Among these events is DNA replication, which is essential for cells to renew themselves. What does cell renewal consist of? Cellular renewal is the capacity of cells to renew themselves.<\/p>","protected":false},"author":8,"featured_media":16752,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[432],"tags":[15,42,651,649,652,647,650],"class_list":["post-16750","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-aprende-genetica","tag-adn","tag-adntro","tag-hebra","tag-helicasa","tag-okazaki","tag-polimerasa","tag-ssb"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Replicaci\u00f3n del ADN<\/title>\n<meta name=\"description\" content=\"La replicaci\u00f3n del ADN es imprescindible para que las c\u00e9lulas se puedan renovar. 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